Elevated levels of 8-iso-prostaglandin F2α in acute coronary syndromes are associated with systemic and local platelet activation

INTRODUCTION: Oxidative stress is an important causative factor in atherosclerosis. Isoprostanes are derivatives of arachidonate oxidized by reactive oxygen species (ROS). Oxidized lipids are markers of oxidative stress, important mediators of atherosclerosis, and activators of platelets. 8-iso-prostaglandin F2α (8-iso-PGF2α) is a stable isoprostane and reliable marker of oxidative stress in vivo. OBJECTIVES: The aim of the study was to determine the level of oxidative stress in acute coronary syndromes (ACS) and its correlations with the para meters of hemo stasis. PATIENTS AND METHODS: Fourty-nine patients aged 46 to 76 years, including 28 with ACS and 25 with stable coronary artery disease (CAD), were enrolled to the study. The levels of 8-iso-PGF2α, soluble CD40 ligand (sCD40L), P-selectin (P-sel), β-thromboglobulin, and the thrombin-antithrombin complex (TAT) in the plasma of venous blood were determined. A microvascular injury model was also used to evaluate TAT generation and sCD40L levels in blood collected every 60 seconds at the site of standardized microvascular injury. RESULTS: 8-iso-PGF2α levels were significantly higher in ACS compared to CAD patients (363.2 ±45.94 vs. 328.2 ±31.96 pg/ml, P = 0.011) and correlated with venous plasma levels of P-sel and β-thromboglobulin in the ACS (r = 0.66; P = 0.0005 and r = 0.62; P = 0.001, respectively) and CAD groups (r = 0.46; P = 0.02 and r = 0.49; P = 0.01, respectively). In the microvascular injury model, the maximum concentrations of sCD40L in the ACS group were associated with plasma 8-iso-PGF2α levels (r = 0.50, P = 0.01). No correlations between 8-iso-PGF2α and markers of thrombin generation in venous blood and microvascular injury model were observed. CONCLUSIONS: Plasma levels of 8-iso-PGF2α are significantly higher in ACS compared with stable CAD and correlate with platelet activation.